Angela Ivask 1, angela.
Abstract This lab was done to test for macromolecules consisting of carbohydrates, lipids, proteins, and nucleic acids by using specific reagents to test for each. The result would bring out a specific color change in the macromolecule. A color change would verify the sample positive for that macromolecule.
An educated guess was made that glucose and sucrose held simple sugars and starch did not. The results concluded this. For starch, iodine was used as a reagent.
The hypothesis was that color would change blue-black on the starch sample and not on the sugar samples. Also, tested was an onion and potato. The hypothesis was that the potato contained starch and the onion did not.
The results of this were also conclusive. For proteins, Ninhydrin and biuret was used as the reagent. Ninhydrin tested for both amino acids and protein. Biuret tests for protein only. For this experiment the only hypothesis was that the starch sample would more than likely not change color.
The results included color changes with Ninhydrin solution on the Amino acid sample and the Albumin sample. Also the color changed for the Albumin sample when tested with Biuret reagent.
For lipids, Sudan III was the reagent used. The hypothesis was that color would change with corn oil. The results concluded this and that egg white samples and honey did not contain lipids. Another test was done for lipids by rubbing substances on an unglazed paper. The oil, lard, and margarine were expected to leave a translucent spot and they did.
Introduction Macromolecules are in all forms of life. These organic compounds are carbohydrates, lipids, proteins, and nucleic acids.LAB REPORT Introduction All living things contain organic molecules such as lipids, carbohydrates, proteins and acids. In order to detect the presence of carbohydrates, proteins and lipids we have to do experiments.
The Iodine test is used to detect the presence of carbohydrates.
Jessie & Zoey Lab Report. Search this site. Browse Our Site. Testing Food for Biological Macromolecules/ NutrientsPictures of Experiment.
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